SQUID to evaluate mitochondrial stress
Mutations to mitochondrial DNA, the generation of reactive oxygen species, and an insufficient supply of oxygen or fuel generate mitochondrial stress. There is no tool to characterize transcriptional and metabolic signatures induced by distinct types of mitochondrial stress.
The mitochondrial stress response is the mechanism by which cells respond to dysfunctional mitochondria.
The researchers describe the characterization of cellular responses to mitochondrial inhibitors and generate a tool, termed SQUID, that can deconvolve signatures of mitochondrial stress from RNA sequencing or metabolic data.
Using SQUID, the authors profiled mitochondrial stress in The Cancer Genome Atlas (TCGA) PanCancer Atlas, identifying a signature of pyruvate import deficiency in IDH1-mutant glioma.
https://www.cell.com/cell-reports-methods/fulltext/S2667-2375(25)00063-3
