The nitrogenase-like enzyme superfamily
Nitrogenases are the only enzymes that reduce dinitrogen to ammonia, a reaction essential for all life on Earth.
Recent work demonstrates that nitrogen fixation‐like (Nfl) enzymes have extensive functions beyond nitrogen fixation, including reducing C=C double bonds of tetrapyrroles and breaking C–S bonds to support bacterial sulfur scavenging.
All nitrogenase-like enzymes consist of a reductase component containing an [Fe4S4]-cluster, and a catalytic component containing diverse metalloclusters.
The catalytic components of the nitrogenase-like enzymes contain simpler FeSclusters than nitrogenases, including [Fe4S4]-clusters and nitrogenase cluster precursors.
https://www.cell.com/trends/biochemical-sciences/fulltext/S0968-0004(25)00277-4
