Plant CRISPR genome editing without tissue culture
Tissue culture-free CRISPR editing overcomes long-standing genotype and regeneration barriers in plant biotechnology.
Meristem-targeted strategies and germline-focused approaches enable efficient, heritable, and often transgene free genome editing.
Viral delivery systems and compact editors (e.g., TnpB) expand editing efficiency and mobility into meristems and reproductive tissues.
These innovations provide genotype independent, scalable platforms for crop improvement across both dicots and monocots.
Future integration with synthetic biology and nanoparticle delivery promises to accelerate precision breeding for sustainable agriculture.
https://www.cell.com/trends/biotechnology/fulltext/S0167-7799(25)00538-4
