Extracellular matrix composition regulation in human corticogenesis

Gene expression is regulated by epigenetic mechanisms during neurogenesis, but the extent of epigenetic remodeling during human cortical development remains unknown.

The researchers in this study used a mass-cytometry-based approach, Epi-CyTOF, for the simultaneous single-cell analysis of more than 30 epigenetic marks. .

The researchers report that epigenetic factors of the Polycomb repressive complex 2 (PRC2)-mediated H3K27me3 regulate neurogenic gene expression programs and show that H3K27me3 enrichment in the human developing neocortex.

PRC2 also regulates extracellular matrix composition in human cortical organoids.

Inhibition PR2  increased production of the ECM proteins Syndecan 1 and laminin alpha 1 and promoted neural progenitor cell differentiation, resulting in smaller brain organoids.

https://www.cell.com/neuron/fulltext/S0896-6273(25)00474-X

https://sciencemission.com/H3K27me3-regulation-of-ECM-composition