Mitochondrial translation monitoring
Mitochondrial translation is important for synthesis of the essential oxidative phosphorylation (OXPHOS) subunits.
The researchers in this study present advanced derivatives of mitochondrial ribosome profiling.
By quantifying initiation, elongation, and ribosome collisions and by dissecting the impacts of tRNA anticodon-loop modifications and the initiation mechanism of internal translons, they uncover dynamic regulation of mammalian mitochondrial translation that ensures cellular energy production.
The study also revealed mitochondrial translation initiation factor (mtIF) 3-mediated translation initiation from internal open reading frames (ORFs) and programmed mitoribosome collision sites across the mitochondrial transcriptome.
https://www.cell.com/molecular-cell/fulltext/S1097-2765(25)00863-9
