Single-cell transcriptomics at isoform resolution
Advances in single-cell RNA sequencing now enable full-length transcript isoform resolution through computational short read reconstruction or direct long-read sequencing.
Continuous improvements in sequencing platforms, experimental workflows, and computational methods are expanding the scale and capability of isoform-resolved single-cell RNA sequencing.
Isoform-resolved single-cell RNA sequencing reveals transcriptomic complexity beyond gene-level expression, encompassing transcript isoforms, nucleotide-resolution variants in RNA, allele-specific expression and transcript structure, transposable element-derived transcripts, fusion transcripts, nonpolyadenylated RNAs, RNA modifications, and immune repertoires.
Emerging dimensions, such as spatial, multiomic, perturbation, population, and AI-driven analyses, are redefining the frontier of isoform-resolved single-cell biology.
https://www.cell.com/trends/genetics/fulltext/S0168-9525(26)00140-X
