Prime editors for mammalian genome editing
The newly developed prime editing system, called pvPE, which uses a reverse transcriptase derived from porcine endogenous retrovirus, outperforms other reported prime editing systems in both editing efficiency and precision.
Multiple optimization strategies boosted editing efficiency by 24.38–101.69-fold compared with the original pvPE across diverse mammalian cell lines.
A small molecule, nocodazole, significantly enhanced pvPE editing efficiency by modulating the DNA repair pathway.
Using the pvPE + Nocodazole system and somatic cell nuclear transfer, we generated triple gene-edited pigs that could serve as a valuable model for research into Alzheimer’s disease in humans.
https://www.cell.com/trends/biotechnology/fulltext/S0167-7799(25)00314-2
