3D human lymphoid model for chronic lymphocytic leukemia (CLL) study
Three-dimensional (3D) in vitro modeling is opening the doors to high-quality ex vivo systems for the study of blood cancers and their interaction with the tissue microenvironment (TME).
The researchers develop a dynamic, gelatin-based, hard porous biomaterial as a support matrix for lymphoid-like model to study chronic lymphocytic leukemia (CLL) physiopathology and show the advantages of the perfusion on 3D cell cultures.
They developed the 3D models of lymph node and bone marrow in bioreactors under dynamic perfusion conditions that promoted a tissue-like internal organization of cells and expression of specific functional markers and deposition of an intricate extracellular matrix protein network.
The authors also observed leukemic cell behavior in CCL cells that circulated in the system and showed expression of key markers involved in tumor progression.
https://www.cell.com/cell-reports-methods/fulltext/S2667-2375(25)00141-9
