A novel biosensor for analysis of STING activation
Spatiotemporal analyses of STING activation in living cells have so far been hampered by the shortage of suitable biosensors.
This study develops the fluorescent reporter SIRF that specifically captures STING activation in response to cGAMP, viral infection, or apoptosis.
The biosensor SIRF allows the monitoring of cellular cGAMP levels in a time- and concentration-dependent manner by simple live-cell imaging and open-source downstream analysis.
SIRF is compatible with single-cell characterisation of the responses to viral infection, mtDNA release upon apoptosis, and other sources of cytoplasmic dsDNA.
SIRF enables spatiotemporal analyses of cGAMP levels across cell populations. They also show that ruptured micronuclei do not activate STING signalling.
https://www.embopress.org/doi/full/10.1038/s44318-025-00370-y
