Single-cell omics sequencing technologies

Single-molecule long-read sequencing (SMS) is advancing rapidly. Sequencing accuracy has progressively improved, with Pacific Biosciences achieving 99.9% accuracy and Oxford Nanopore Technologies reaching over 99%. 

SMS platform-based single-cell transcriptome sequencing directly identifies full-length RNA isoforms, enabling the study of complex alternative splicing events at single-cell resolution.

SMS-based single-cell genome sequencing technologies generate long reads ranging from 6 to 10 kb, enabling genome assembly and whole-chromosome-scale haplotype phasing with fewer than 100 single cells.

SMS-based single-cell epigenome sequencing technologies enable the detection of chromatin accessibility, histone modifications, TF binding sites, and 3D genome architecture.

These methods facilitate the resolution of allele-specific epigenetic modifications, epigenetic states in complex genomic regions such as repetitive elements, and ‘dark genome’ features such as higher-order chromatin interactions.

https://www.cell.com/trends/genetics/fulltext/S0168-9525(25)00196-9

https://sciencemission.com/Single-cell-omics-sequencing-technologies