Whole-genome spatial transcriptomics with no sequencing
Spatial transcriptomics tools currently available are still limited in either transcriptomic coverage or spatial resolution, hindering unbiased, hypothesis-free transcriptomic analyses at high spatial resolution.
The researchers developed reverse-padlock amplicon-encoding fluorescence in situ hybridization (RAEFISH) tool.
It delivers whole-transcriptome (>20,000 genes) imaging from human or mouse genes at single molecule resolution in cells and tissues.
The technology also enables direct gRNA detection for high-content, image-based CRISPR screens.
